1. Before taking preventive measures by using Allergoff® Spray, it was found that the concentration level of the main mite D. pteronyssinus (Der p 1) allergen in the dust samples from the mattress was high in most cases (the highest value is 0.802 μg / g) or medium from 0.2 – 0.4 μg / g. Only in individual cases the concentration of Der p 1 allergen has been found low as 1 μg / g or at the borderline of detection of 0.009 μg / g.
2. After Allergoff® Spray has been used, the test has been repeated. The results showed the absence of the main Der p 1 allergen in the samples of collected mattress dust, which was analyzed using colored test called “Acarex test” in all patients (100%). On the other hand, based on the detailed allergen detection in the second stage of the study, the average concentration of Der p 1 in 1 gram of the examined dust decreased significantly to 0.176 ± 0.066 μg (Table 2 – the table does not include samples that do not allow to determine the level of allergen in collected dust samples).
3. The demonstrated decrease in the content of the main allergen of D. pteronyssinus mite was correlated with the patients’ subjective assessment during the medical questionnaire. In the first stage, the patients indicated a significant reduction in disease symptoms by an average of 5.4 ± 0.8 (± standard deviation) points on a 10 point scale in relation to the baseline test, where full symptom score was 10 points and 0 indicated complete resolution. This result was statistically significant (p <0.05).
The second stage showed a correlation between decrease in the Der p 1 allergen concentration and the improvement of the patients’ well-being (improvement of the clinical picture expressed by the reduction of nasal symptoms): R = -0.7 for p <0.05 (graph 1).